Development of a CZE method for the determination of Olmesartan medoxomil in tablets


ÇELEBİER M. , ALTINÖZ S.

CHROMATOGRAPHIA, cilt.66, ss.929-933, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 66
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1365/s10337-007-0424-2
  • Dergi Adı: CHROMATOGRAPHIA
  • Sayfa Sayıları: ss.929-933

Özet

A CZE method was developed and validated for the analysis of Olmesartan medoxomil (OLMD) in tablets. The influences of pH, buffer concentration, applied voltage and capillary temperature on the migration time of OLMD were investigated. About 50 mM pH 6.5 phosphate buffer were used as background electrolyte. The optimum instrument parameters were found to be 30 degrees C temperature with 30 kV applied voltage and diode array detection was carried out at 210 nm. OLMD was hydrodynamically injected (P-inj = 50 mbar, t(inj) = 3 s) and an internal standard, diflunisal (IS), was used to improve the precision and repeatability. Under these conditions, the migration time of OLMD was 2.32 min and the total analysis time was shorter than 5 min. Linearity range for the developed method was found to be 2.0-50.0 mu g mL(-1) and the limit of detection was 0.5 mu g mL(-1). The developed method was applied for the analysis of OLMD in pharmaceutical tablet formulations.