Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction


Arisoy M., Aysev D., Ekim M., Ozel D., Kose S., Ozsoy E., ...More

INTERNATIONAL JOURNAL OF CLINICAL PRACTICE, vol.60, no.2, pp.170-173, 2006 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 60 Issue: 2
  • Publication Date: 2006
  • Doi Number: 10.1111/j.1368-5031.2005.00668.x
  • Title of Journal : INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
  • Page Numbers: pp.170-173

Abstract

A total of 161 Escherichia coli (E. coli) strains isolated from children with urinary tract infection (UTI) were analysed for the genes encoding the virulence factors such as pyelonephritis (pap), s fimbriae (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotising factor I (cnf I) and aerobactin (aer) by multiplex PCR. Ninety-four E. coli strains were found to carry at least one virulence factor. Therefore, 58.38% of total population was positive for one virulence gene at least. Percentage of genes within the total population for pap, sfa, afaI, hly, cnf I and aer was found as 22.98, 6.21, 9.94, 1.24, 9.94 and 39.75, respectively. Our analysis showed that sfa-pap (p < 0.001); pap-aer, afaI-aer and cnf I-pap (P < 0.05) and hly-sfa (p < 0.01) significantly co-occurred in their respective samples. In the light of these findings, we suggest an important role of pap causing UTI.