Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) cryogel discs

Gokturk I., Percin I., DENİZLİ A.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, vol.46, no.6, pp.602-609, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.1080/10826068.2015.1085400
  • Journal Name: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.602-609
  • Keywords: Catalase, cryogel disc, enzyme, glutamic acid, IMAC, purification, ION AFFINITY-CHROMATOGRAPHY, METHACRYLOYLAMIDOGLUTAMIC ACID, MAGNETIC BEADS, UO22+ REMOVAL, IMMOBILIZATION, ADSORPTION, PROTEINS, COLUMNS, MATRIX, ENZYME
  • Hacettepe University Affiliated: Yes

Abstract

In this study, iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) (PHEMAGA/Fe3+) cryogel discs were prepared. The PHEMAGA/Fe3+ cryogel discs were characterized by elemental analysis, scanning electron microscopy, Fourier transform infrared spectroscopy, swelling tests, and surface area measurements. The PHEMAGA/Fe3+ cryogel discs had large pores ranging from 10 to 100 mu m with a swelling degree of 9.36 g H2O/g cryogel. Effects of pH, temperature, initial catalase concentration, and flow rate on adsorption capacity of the PHEMAGA/Fe3+ cryogel discs were investigated. Maximum catalase adsorption capacity (62.6 mg/g) was obtained at pH 7.0, 25 degrees C, and 3 mg/ml initial catalase concentration. The PHEMAGA/Fe3+ cryogel discs were also tested for the purification of catalase from rat liver. After tissue homogenization, purification of catalase was performed using the PHEMAGA/Fe3+ cryogel discs and catalase was obtained with a yield of 54.34 and 16.67 purification fold.