The purpose of this study is to develop a scale to measure attitude towards gender based choice of profession for female high school students. The subjects of this study were girls from 9 and 12 high schools which are from different socio-economic levels. In this study, firstly data collected from 259 students' entered into SPSS. Explanatory and confirmatory factor analysis was used in order to identify the validity of Attitude Towards Gender Based Choice of Profession Scale (ATGBCPS). The results of explanatory factor analysis showed that the construct of this scale has 24 items and 7 factors and it explain 60.019 % of total variance. Confirmatory factor analysis supported these results. The reliability of the scale was tested with Cronbach-Alpha coefficient and split-half method by applying the scale to 174 students. Cronbach-Alpha reliability coefficient was found as .853 and the test-retest reliability coefficient found as .812. The findings of the study shows that ATGBCPS is able to measure attitude towards gender based choice of profession for female high school students.