Detection of ciprofloxacin through surface plasmon resonance nanosensor with specific recognition sites


Sari E., ÜZEK R., DUMAN M., DENİZLİ A.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.29, no.11, pp.1302-1318, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 11
  • Publication Date: 2018
  • Doi Number: 10.1080/09205063.2018.1457417
  • Journal Name: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1302-1318
  • Keywords: Ciprofloxacin, nanosensor, surface plasmon resonance, molecular imprinting, miniemulsion, MOLECULARLY IMPRINTED POLYMERS, REAL-TIME DETECTION, ENZYMATIC ROTATING BIOSENSOR, SOLID-PHASE EXTRACTION, SYNTHETIC WASTE-WATER, LABEL-FREE DETECTION, CAPILLARY-ELECTROPHORESIS, METABOLITE CIPROFLOXACIN, SENSITIVE DETECTION, PASTE ELECTRODE
  • Hacettepe University Affiliated: Yes

Abstract

The novel nanosensor based on Surface Plasmon Resonance (SPR) was developed for sensitive and selective detection of ciprofloxacin via molecularly imprinted nanoparticles (MIP/NPs). NPs were synthesized through miniemulsion polymerization technique with methacrylic acid as a functional monomer. FTIR, SEM, zetasizer and contact angle measurements were used for the characterization of MIP/NPs. After modification the SPR chip surface, the nanosensor was used for detection of ciprofloxacin in aqueous solution. According to selected concentration range, the correlation coefficient and limit of detections were obtained as 0.993 (R-2) and 3.21 and 7.1ppb in ultrapure water and SWW, respectively. Association kinetic analysis, Scatchard, Freundlich, Langmuir and Freundlich-Langmuir isotherms were also performed on the data to investigate adsorption behaviour of ciprofloxacin on the surface of nanosensor. Tetracycline and enrofloxacin were used as competitor agents to examine the selectivity of nanosensor. Performance of the SPR nanosensor was also investigated by using synthetic wastewater (SWW) for detection of CPX. The reusability of nanosensor was investigated and good repeatability was obtained with 5.81% RSD. As a result, selective, simple and low-cost method to detect ciprofloxacin in aqueous solution was developed by combining MIP/NPs and SPR.