Local and systemic angiogenic and antiangiogenic response in rats after 70% hepatectomy


SARSENOV D., DOĞRUL A. B. , KÖSEMEHMETOĞLU K. , TIRNAKSIZ M. B. , ABBASOĞLU O.

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, vol.10, no.3, pp.2939-2949, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 3
  • Publication Date: 2017
  • Title of Journal : INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
  • Page Numbers: pp.2939-2949

Abstract

Angiogenesis accompanying liver regeneration is regulated by both proangiogenic and anti-angiogenic effectors. This study investigated angiogenesis at both local and systemic levels also targeting measurement of endostatin's local expression during hepatic regeneration. Methods: 64 female rats underwent 70% hepatectomy and were sacrificed on the posthepatectomy days 1, 3, 5, 7, 10, 14 and 21. Local angiogenesis and anti-angiogenesis were defined by immunoreactivity to VEGFR-2 and endostatin respectively. Systemic VEGF and endostatin were identified by ELISA with corresponding antibodies. Results: Both VEGF and VEGFR-2 began to rise on day 3. On day 5 VEGF reached local peak with 260.60 pg/ ml while VEGFR-2 expression became 3.63 degrees. At day 21 both parameters returned to corresponding basal levels. Peak values of systemic endostatin (68.83 ng/ml) were detected on day 7. Local endostatin expression in tissue was found to be high through groups from 7th to 14th days. On day 14 through 21 when tissue endostatin went decreasing systemic one stayed high. Conclusions: throughout liver regeneration expression of endostatin in extracellular matrix was high and related to angiogenesis. Systemic presence of endostatin was correlating with in situ expression of endostatin which should play a role in regulation of endocrine effects of angiogenic factors like VEGF.