Proliferation and monoclonal antibody production of hybridoma cells immobilized on non-woven polyester fabric discs

Aslankaraoglu E., Gumusderelioglu M., Yilmaz S., Bingol N.

15th Annual Meeting of the Japanese-Association-for-Animal-Cell-Technology, Fuchu, Japan, 11 - 15 November 2002, pp.197-201 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Fuchu
  • Country: Japan
  • Page Numbers: pp.197-201
  • Hacettepe University Affiliated: Yes


Mouse hybridoma cells producing monoclonal antibody against the 1465 antigenic particles of O type apthhoviruses were proliferated on the non-woven polyester fabric (NWPF) discs in stationary and stirred conditions. Firstly, a series of batch experiments were performed in Petri dishes with and without NWPF disc, containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. The results showed that in the presence of the NWPF discs cell viability, cell yield and total monoclonal antibody production was higher than that of the disc-free culture. In the second part, reactor cultures were realized in a 1L spinner basket containing NWPF discs. During the culture the cell growth and MAb production were examined. The highest MAb concentration were obtained in spinner basket. All results indicated that NWPF-containing spinner basket can be used for the large-scale production of MAb.