Characterization of Propolis Extracts Prepared Using Different Solvents at the Different Concentrations

ÖZKÖK A., Keskin M., Samanci A. E. T., Onder E. Y., TAKMA Ç.

PROGRESS IN NUTRITION, vol.23, no.3, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.23751/pn.v23i3.10795
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
  • Keywords: antioxidant, ethanol extract, flavonoid, glycol extract, phenolic, propolis, ANTIOXIDANT CAPACITY, STANDARDIZATION
  • Hacettepe University Affiliated: Yes


Propolis is an important bee product thanks to having many biological activities such as antibacterial, antiviral, antifungal. However, it has a complex structure as it contains many components such as beeswax, balm, pollen, flavonoids, and phenolic acids. Useful components of this structure must be dissolved in an effective and also a reliable solvent in order to be released. In this study, propolis samples were collected from different regions of Turkey and these samples dissolved in various solvents with different concentrations (70% glycol was used for water-soluble propolis extracts of 10% and 15%, and 70% ethanol was used for ethanol extracts of 20% and 30%) for the preparing extracts. Then, in these extracts, dry matter, total phenolic, total flavonoid and antioxidant capacity were determined and compared to the each other. A statistically significant difference was found between the results of all the concentrations examined in this study (p<0.01). In addition, as a result of the results obtained, 20% ethanol extract (70% v/v ethanol) and 15% glycol propolis extract (70% v/v glycol) are statistically similar; and 30% ethanol propolis extract (70% v/v ethanol) was found to contain the most bioactive components (p<0.01). With this study, it has been revealed that propolis, which differs in botanical origin and component content, can be prepared in certain standards depending on the solvent type and concentrations. Due to the fact that there is very little study and literature knowledge on this subject within our knowledge, these results can be useful of the basic standardization of the propolis.