Targeted mesoporous silica nanoparticles for improved inhibition of disinfectant resistant Listeria monocytogenes and lower environmental pollution


Sudagidan M., Yildiz G., Onen S., Al R., Temiz S. S. N. , Yurt M. N. Z. , ...More

JOURNAL OF HAZARDOUS MATERIALS, vol.418, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 418
  • Publication Date: 2021
  • Doi Number: 10.1016/j.jhazmat.2021.126364
  • Journal Name: JOURNAL OF HAZARDOUS MATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Disinfectants, Resistant pathogen bacteria, Aptamers, Nanoparticles, Listeria, BENZALKONIUM-CHLORIDE RESISTANCE, QUATERNARY AMMONIUM-COMPOUNDS, STRAINS, APTAMER, ANTIBACTERIAL, FISH
  • Hacettepe University Affiliated: Yes

Abstract

Benzalkonium chloride (BAC) is a common ingredient of disinfectants used for industrial, medical, food safety and domestic applications. It is a common pollutant detected in surface and wastewaters to induce adverse effects on Human health as well as aquatic and terrestrial life forms. Since disinfectant use is essential in combatting against microorganisms, the best approach to reduce ecotoxicity level is to restrict BAC use. We report here that encapsulation of BAC in mesoporous silica nanoparticles can provide an efficient strategy for inhibition of mi-crobial activity with lower than usual concentrations of disinfectants. As a proof-of-concept, Listeria mono-cytogenes was evaluated for minimum inhibitory concentration (MIC) of nanomaterial encapsulated BAC. Aptamer molecular gate structures provided a specific targeting of the disinfectant to Listeria cells, leading to high BAC concentrations around bacterial cells, but significantly reduced amounts in total. This strategy allowed to inhibition of BAC resistant Listeria strains with 8 times less the usual disinfectant dose. BAC encapsulated and aptamer functionalized silica nanoparticles (AptBACNP) effectively killed only target bacteria L. monocytogenes, but not the non-target cells, Staphylococcus aureus or Escherichia coli. AptBACNP was not cytotoxic to Human cells as determined by in vitro viability assays.