Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

Karaoz E., Aksoy A., Ayhan S., Sariboyaci A. E., Kaymaz F., KASAP M.

HISTOCHEMISTRY AND CELL BIOLOGY, cilt.132, sa.5, ss.533-546, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 132 Sayı: 5
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s00418-009-0629-6
  • Dergi Adı: HISTOCHEMISTRY AND CELL BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.533-546
  • Anahtar Kelimeler: Rat, Bone marrow, Stem cell, Differentiation, Proliferation, Ultrastructure, Immunophenotype, STROMAL CELLS, EXPRESSION, SCAFFOLD, CULTURE, NESTIN
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and alpha-SMA), neurogenic (gamma-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.