Anti-inflammatory and cytotoxic activities of five Veronica species

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HARPUT U., Saracoglu I., INOUE M., OGIHARA Y.

BIOLOGICAL & PHARMACEUTICAL BULLETIN, vol.25, no.4, pp.483-486, 2002 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 4
  • Publication Date: 2002
  • Doi Number: 10.1248/bpb.25.483
  • Journal Name: BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.483-486
  • Keywords: Veronica species, Scrophulariaceae, nitric oxide, macrophages, cytotoxicity, 2,2-diphenyl-1-pieryl-hydrazyl (DPPH), IRIDOID GLUCOSIDES, ANAGALLIS-AQUATICA
  • Hacettepe University Affiliated: Yes

Abstract

Biological activities of five Veronica species (Scrophulariaceae), V. cymbalaria, V. hederifolia, V. pectinata var. glandulosa, V. persica and V. polita were studied for their anti-inflammatory and cytotoxic activities. Their methanol extracts showed both the inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated macrophages and cytotoxic activity against KB epidermoid carcinoma and B16 melanoma. When the methanol extracts were fractionated between water and chloroform, water fractions significantly inhibited NO production without any cytotoxicity, while chloroform fractions showed cytotoxicity dose-dependently. When the radical scavenging activity was determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH), water fractions of the five Veronica species scavenged free radicals effectively, suggesting that the inhibitory effect of this species on NO production was due to their radical scavenging activity. On the other hand, chloroform fractions of Veronica species except for V. cymbalaria showed similar cytotoxic activity against KB and B16 melanoma cells.