A novel two-enzyme amperometric electrode for lactose determination

Gulce H., Gulce A., Yildiz A.

ANALYTICAL SCIENCES, cilt.18, ss.147-150, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 18 Konu: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.2116/analsci.18.147
  • Dergi Adı: ANALYTICAL SCIENCES
  • Sayfa Sayıları: ss.147-150

Özet

Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.