A novel two-enzyme amperometric electrode for lactose determination


Gulce H., Gulce A., Yildiz A.

ANALYTICAL SCIENCES, cilt.18, sa.2, ss.147-150, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.2116/analsci.18.147
  • Dergi Adı: ANALYTICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-150
  • Hacettepe Üniversitesi Adresli: Hayır

Özet

Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.