S-citalopram imprinted monolithic columns for capillary electrochromatography enantioseparations

Derazshamshir A., GÖKTÜRK BAŞAL I., YILMAZ F., DENİZLİ A.

ELECTROPHORESIS, cilt.42, sa.24, ss.2672-2682, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 24
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/elps.202100222
  • Dergi Adı: ELECTROPHORESIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2672-2682
  • Anahtar Kelimeler: Antidepressant, Capillary electrochromatography, Chirality, Molecular imprinting, Monolithic column, ENANTIOMERIC SEPARATION, CHIRAL RECOGNITION, BETA-CYCLODEXTRIN, HUMAN PLASMA, RACEMIC CITALOPRAM, STATIONARY-PHASE, AMINO-ACIDS, DRUGS, ELECTROPHORESIS, ESCITALOPRAM
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In this study, the molecular imprinting method was used to separate enantiomeric forms of chiral antidepressant drug, R,S-citalopram (R,S-CIT) in aqueous solution by CEC system combining the advantages of capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). For that, an amino acid-based molecularly imprinted monolithic capillary column was designed and used as a stationary phase for selective separation of S-citalopram (S-CIT) for the first time. S-CIT was selectively separated from the aqueous solution containing the other enantiomeric form of R-CIT, which is the same in size and shape as the template molecule. Morphology of the molecularly imprinted (MIP S-CIT) and non-imprinted (NIP S-CIT) monolithic capillary columns was observed by scanning electron microscopy. Imprinting efficiency of MIP S-CIT monolithic capillary column used for selective S-CIT separation was verified by comparing with NIP S-CIT and calculated imprinting factor (I.F:1.81) proved the high selectivity of the MIP S-CIT for S-CIT. Cavities formed for S-CIT form enabled selective (alpha = 2.08) separation of the target molecule from the other enantiomeric R-CIT form. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 7.68 x 10(-6) m(2)/Vs for R,S-CIT at pH 7.0 10 mM PB and 50% ACN ratio. The performance of both MIP S-CIT and NIP S-CIT columns was estimated by repeating the R,S-CIT separations with intra-batch and inter-batch studies for reproducibility of retention times of R,S-CITs. Estimated RSD values that are lower than 2% suggest that the monolithic columns separate R,S-CIT enantiomers without losing separation efficiency.