Akademik Veri Yönetim Sistemi
8th Turkish Society of Toxicology Congress, Antalya, Türkiye, 15 - 18 Kasım 2012, ss.40
This study describes the
optimization and validation of a quick and simple method for chrome (Cr) and nickel
(Ni) levels in placenta samples by graphite furnace atomic absorption
spectrometry (GF-AAS), which has been proved to be useful for toxicological
research. Ni is generally considered as a hazardous material, but at the same
time, it is nowadays recognized as an essential element. Furthermore, it holds
a special place among the heavy metals. Cr(III) is a trace element species
essential for the proper functioning of living
organisms but Cr(VI) has toxic effects on biological systems and
classified as a Group 1 human carcinogen. The determination of Cr and Ni levels
in biological samples by GF-AAS is the most widely employed techniques, because of its relatively low cost, sufficiently
sensitive and high detection limits. A microwave-assisted wet acid digestion
procedure was developed as a sample pretreatment. This method was validated
with Certified Reference Materials (NC SZC 73016-Chicken) for the evaluation of
analytical results. High accuracy was obtained for Cr and Ni in the matrix of
total digestion, where losses of the analyte could be attributed to sample
treatment with HNO3. The Mean levels of placenta-Cr and Ni were
found as 220,66 ppb (ranging from 31.96 ppb to 913.51 ppb) and 124,24 ppb
(ranging from 24.41 ppb to 683.22 ppb), respectively. These results will be
discussed according to age, gestational age, smoking habits, and consuming
seafood of participants