"Assessment of Cr and Ni Levels in Placenta by Graphite Furnace Atomic Absorption Spectrometry"


Arıca E., Eren Z. S., Kartal Y., Türksoy V. A., Söylemezoğlu T.

8th Turkish Society of Toxicology Congress, Antalya, Turkey, 15 - 18 November 2012, pp.40

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.40
  • Hacettepe University Affiliated: Yes

Abstract

This study describes the optimization and validation of a quick and simple method for chrome (Cr) and nickel (Ni) levels in placenta samples by graphite furnace atomic absorption spectrometry (GF-AAS), which has been proved to be useful for toxicological research. Ni is generally considered as a hazardous material, but at the same time, it is nowadays recognized as an essential element. Furthermore, it holds a special place among the heavy metals. Cr(III) is a trace element species essential for the proper functioning of living  organisms but Cr(VI) has toxic effects on biological systems and classified as a Group 1 human carcinogen. The determination of Cr and Ni levels in biological samples by GF-AAS is the most widely employed techniques, because of its relatively low cost, sufficiently sensitive and high detection limits. A microwave-assisted wet acid digestion procedure was developed as a sample pretreatment. This method was validated with Certified Reference Materials (NC SZC 73016-Chicken) for the evaluation of analytical results. High accuracy was obtained for Cr and Ni in the matrix of total digestion, where losses of the analyte could be attributed to sample treatment with HNO3. The Mean levels of placenta-Cr and Ni were found as 220,66 ppb (ranging from 31.96 ppb to 913.51 ppb) and 124,24 ppb (ranging from 24.41 ppb to 683.22 ppb), respectively. These results will be discussed according to age, gestational age, smoking habits, and consuming seafood of participants