Development of tumor-specific liposomes containing quantum dots-photosensitizer conjugate used for radiotherapy

Karabuga M., Erdogan S., Timur S. S., VURAL İ., calamak S., Ulubayram K.

JOURNAL OF LIPOSOME RESEARCH, cilt.32, sa.4, ss.396-404, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 4
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/08982104.2022.2087082
  • Dergi Adı: JOURNAL OF LIPOSOME RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.396-404
  • Anahtar Kelimeler: Liposome, radiosensitizer, quantum dot, chlorine-e6, radiotherapy, PHOTODYNAMIC THERAPY, IN-VIVO, NANOPARTICLES, COMPLEXES, DELIVERY, RADIATION, CELLS
  • Hacettepe Üniversitesi Adresli: Evet

Özet

This study aims to develop a multifunctional liposomal radiosensitizer to destroy more tumor cells by using lower radiation doses compared to clinically used 6 MV X-ray doses. To achieve this aim, first Chlorine-e6 (Ce6) was covalently bound to functional groups of outer surfaces of quantum dots (QDs) through EDC/NHS reactions. Then, QDs-Ce6 conjugate loaded, nanosized, PEG-coated, and tumor-specific folic acid-modified immunoliposome dispersions were prepared by film method. Enhanced anti-proliferation activity of free and liposomal conjugate against 4T1 (murine breast cancer) cell lines was investigated at different X-ray doses (5, 10, 15, and 20 Gy). As a result, the best radiosensitizer effect was observed at a 5 Gy X-ray dose and it was found that following the X-ray irradiation, immunoliposome dispersions containing QDs-Ce6 conjugate killed 26.8 +/- 1.7% more cancer cells than radiation alone.