ENZYME AND MICROBIAL TECHNOLOGY, vol.45, no.1, pp.15-21, 2009 (SCI-Expanded)
The combined effects of initial sucrose and initial Remazol Turquoise Blue-G (RTBG) reactive dye concentrations on the specific growth rate and dye bioaccumulation efficiency of Candida utilis was investigated and optimized using response surface methodology (RSM) in this study. A 2(2) full factorial central composite design was successfully used for experimental design and analyses of the results. Two numerical correlations fitted to a second-order quadratic equation were obtained to estimate the responses of specific growth rate and dye uptake yield. The statistical analysis indicated that both the microbial growth and removal yield of dye enhanced with raising Sucrose concentration up to 15 gl(-1) and diminished with the increase in initial RTBG dye concentration up to approximately 500 mgl(-1) due to inhibition caused by high concentrations of RTBG dye. The optimum combination predicted via RSM confirmed that C. utilis was capable of bioaccumulating RTBG with the maximum uptake yield of 82.0% in 15 gl(-1) sucrose and 50 mg l(-1) dye containing growth medium. (C) 2009 Elsevier Inc. All rights reserved.