Measurement of shear-induced diffusion of red blood cells using dynamic light scattering-optical coherence tomography

Tang J., Erdener S. E., Li B., Fu B., Sakadzic S., Carp S. A., ...More

Conference on Neural Imaging and Sensing, San-Francisco, Costa Rica, 29 - 30 January 2018, vol.10481 identifier identifier

  • Publication Type: Conference Paper / Full Text
  • Volume: 10481
  • Doi Number: 10.1117/12.2290345
  • City: San-Francisco
  • Country: Costa Rica
  • Hacettepe University Affiliated: Yes

Abstract

Dynamic Light Scattering-Optical Coherence Tomography (DLS-OCT) takes the advantages of using DLS to measure particle flow and diffusion within an OCT resolution-constrained 3D volume, enabling the simultaneous measurements of absolute RBC velocity and diffusion coefficient with high spatial resolution. In this work, we applied DLS-OCT to measure both RBC velocity and the shear-induced diffusion coefficient within penetrating venules of the somatosensory cortex of anesthetized mice. Blood flow laminar profile measurements indicate a blunted laminar flow profile, and the degree of blunting decreases with increasing vessel diameter. The measured shear-induced diffusion coefficient was proportional to the flow shear rate with a magnitude of similar to 0.1 to 0.5 x 10(-6) mm(2). These results provide important experimental support for the recent theoretical explanation for why DCS is dominantly sensitive to RBC diffusive motion.