Synthesis and characterization of amino acid containing Cu(II) chelated nanoparticles for lysozyme adsorption


Baydemir G., Andac M., Derazshamshir A., Uygun D. A., Ozcaliskan E., AKGÖL S., ...More

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.33, no.1, pp.532-536, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.1016/j.msec.2012.09.027
  • Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.532-536
  • Keywords: Lysozyme, Nanoparticles, IMAC, Tryptophan, HEN EGG-WHITE, MAGNETIC-FIELD, AFFINITY-CHROMATOGRAPHY, HYDROPHOBIC BEADS, IMMUNOGLOBULIN-G, PURIFICATION, ADSORBENT, MEMBRANES, PROTEINS, SYSTEMS
  • Hacettepe University Affiliated: Yes

Abstract

Immobilized metal ion affinity chromatography (IMAC) is a useful method for adsorption of proteins that have an affinity for transition metal ions. In this study, poly( hydroxyethyl methacrylate-methacryloyl-L-tryptophan) (PHEMATrp) nanoparticles were prepared by surfactant free emulsion polymerization. Then, Cu(II) ions were chelated on the PHEMATrp nanoparticles to be used in lysozyme adsorption studies in batch system. The maximum lysozyme adsorption capacity of the PHEMATrp nanoparticles was found to be 326.9 mg/g polymer at pH 7.0. The nonspecific lysozyme adsorption onto the PHEMA nanoparticles was negligible. In terms of protein desorption, it was observed that adsorbed lysozyme was readily desorbed in medium containing 1.0 M NaCl. The results showed that the metal-chelated PHEMATrp nanoparticles can be considered as a good adsorbent for lysozyme purification. (C) 2012 Elsevier B.V. All rights reserved.