Alkaline-Phosphatase-Based Nanostructure Assemblies for Electrochemical Detection of microRNAs

Voccia D., Bettazzi E., Baydemir G., Palchetti I.

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, cilt.15, sa.5, ss.3378-3384, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 5
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1166/jnn.2015.10201
  • Dergi Adı: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3378-3384
  • Hacettepe Üniversitesi Adresli: Hayır

Özet

Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzyme-decorated liposomes are used as labels to amplify the nnicroRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.