Chronic Cranial Windows for Long Term Multimodal Neurovascular Imaging in Mice

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Kilic K., Desjardins M., Tang J., Thunemann M., Sunil S., Erdener S. E., ...Daha Fazla

FRONTIERS IN PHYSIOLOGY, cilt.11, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3389/fphys.2020.612678
  • Dergi Adı: FRONTIERS IN PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, Directory of Open Access Journals
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Chronic cranial windows allow for longitudinal brain imaging experiments in awake, behaving mice. Different imaging technologies have their unique advantages and combining multiple imaging modalities offers measurements of a wide spectrum of neuronal, glial, vascular, and metabolic parameters needed for comprehensive investigation of physiological and pathophysiological mechanisms. Here, we detail a suite of surgical techniques for installation of different cranial windows targeted for specific imaging technologies and their combination. Following these techniques and practices will yield higher experimental success and reproducibility of results.