Chronic Cranial Windows for Long Term Multimodal Neurovascular Imaging in Mice


Kilic K., Desjardins M., Tang J. , Thunemann M., Sunil S., Erdener S. E. , ...Daha Fazla

FRONTIERS IN PHYSIOLOGY, cilt.11, 2021 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3389/fphys.2020.612678
  • Dergi Adı: FRONTIERS IN PHYSIOLOGY

Özet

Chronic cranial windows allow for longitudinal brain imaging experiments in awake, behaving mice. Different imaging technologies have their unique advantages and combining multiple imaging modalities offers measurements of a wide spectrum of neuronal, glial, vascular, and metabolic parameters needed for comprehensive investigation of physiological and pathophysiological mechanisms. Here, we detail a suite of surgical techniques for installation of different cranial windows targeted for specific imaging technologies and their combination. Following these techniques and practices will yield higher experimental success and reproducibility of results.