Increased endoglin levels correlated with angiogenesis-associated angiopoietin-2 in haemophilia patients


Karaman K., Celik A., AYTAÇ EYÜPOĞLU Ş. S., Bakar-Ates F.

HAEMOPHILIA, vol.27, no.6, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1111/hae.14430
  • Journal Name: HAEMOPHILIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, MEDLINE
  • Keywords: angiogenesis, angiopoietin, cytokine, endoglin, gene expression, haemophilia, PLASMINOGEN-ACTIVATOR INHIBITOR-1, COLLAGEN-INDUCED ARTHRITIS, ANNEXIN-V, ENDOTHELIAL-CELLS, CARTILAGE, BINDING, INTERLEUKIN-4, PHOSPHATIDYLSERINE, HYPOXIA, PATHWAY
  • Hacettepe University Affiliated: Yes

Abstract

Introduction Haemophilia is a bleeding disorder that occurs due to the deficiency of coagulation factors, and the angiogenesis process is an important process underlying the pathophysiology of haemophilic arthropathy. The role of the new adipocytokine endoglin (ENG) in patients with haemophilia is not yet known. Aim The aim of this study is to evaluate the association between ENG protein and angiogenesis-related cytokines in patients with haemophilia for the first time. Methods Plasma protein levels and mRNA expressions of ENG and various angiogenesis-associated cytokines were compared in blood samples collected from 28 patients with haemophilia A or B and 29 healthy volunteers. The relationship between the cytokines and ENG were determined by correlation analysis. Results Plasma ENG levels and angiogenic markers were found to be significantly higher in patients with haemophilia compared to controls. Real-time PCR studies showed that mRNA expressions of ENG, vascular endothelial growth factor A, hypoxia-inducible factor A, and prostaglandin E2 increased in patients with haemophilia. Correlation analysis showed a significant positive correlation between ENG and angiopoietin-2 levels in the haemophilia group. Besides, a significant decrease in annexin-V binding to platelets in haemophilia patients compared to control was found to be related to the bleeding profiles in the patients. Conclusions This study determined that ENG protein may be involved in the formation of angiogenesis in haemophilia patients and its effects may be related to angiogenetic marker angiopoietin-2 in this process. Our findings contribute to the literature during the determination of target proteins in haemophilia treatment.