Preparation and characterization of cyclodextrin nanosponges for organic toxic molecule removal

VARAN C., Anceschi A., Sevli S., Bruni N., Giraudo L., BİLGİÇ E., ...Daha Fazla

INTERNATIONAL JOURNAL OF PHARMACEUTICS, cilt.585, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 585
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.ijpharm.2020.119485
  • Dergi Adı: INTERNATIONAL JOURNAL OF PHARMACEUTICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Chronic kidney disease (CKD), Cyclodextrin (CD), Gastrointestinal (GI), Indole, Nanosponge (NS), Toxic molecule removal, CHRONIC KIDNEY-DISEASE, BETA-CYCLODEXTRIN, DRUG-RELEASE, DELIVERY, FORMULATION, AST-120, DEATH
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Cyclodextrin-based nanosponges (CD-NS) are considered as safe and biocompatible systems for removing toxic molecules from the body. Rapid removal of toxic molecules that are formed in the body from certain food constituents, is relevant especially for patients affected by chronic kidney disease. Within the scope of this study, innovative cyclodextrin polymers were synthesized to form nanosponges able to remove indole, before it could form the toxic indoxyl sulfate in the body. Furthermore, in vivo studies were carried out using the two optimal CD-NS formulations by assessing physicochemical properties, stability, indole adsorption capacity and in vitro cytotoxicity. NS prepared from beta-cyclodextrin cross-linked with toluene diisocyanate was found to be the most effective NS with an in vitro indole adsorption capacity of over 90%. In addition, this derivative was more stable in gastrointestinal media. Animal studies further revealed that oral CD-NSs did not tend to accumulate and damage gastrointestinal tissues and are excreted from the GI tract with minimal absorption. In conclusion, this study suggests that CD-NS formulations are effective and safe in removing toxic molecules from the body. Their potential use in veterinary or human medicine could reduce dialysis frequency and avoid hepatic and cardiac toxicity avoiding the indole formation.