Novel Strategies for Synthesis and Assessment of Dextran Conjugated 9Z,11E Linoleic Acid on Cancer Cell Lines Viability via Wnt/Beta-Catenin Signaling Pathway and Apoptosis

SEVİM AKAN H., DÖNMEZ H. G., Uysaler B., KAPLAN CAN H.

STARCH-STARKE, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1002/star.202300281
  • Dergi Adı: STARCH-STARKE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Analytical Abstracts, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Conjugated linoleic acid (CLA) is one of the positional and structural isomers of linoleic acid. Ninety percent of the CLA contained in natural foods is the cis-9(9Z), trans-11(11E) isomer. CLA also has anti-carcinogenic, anti-adipogenic, and anti-inflammatory properties. Limited studies aim to elucidate the impact of 9Z,11E-CLA on Wnt/beta-catenin signaling.In this study, we have selected MDA-MB-231, T98G, and SAOS-2 cell lines of various cancer origins and investigate the impact of dextran conjugated 9Z,11E CLA (Dex-9Z,11E-CLA) using cell proliferation assay and immunocytochemical methods. Cytotoxic effects of Dex conjugated 9Z,11E CLA are assessed with the MTT method. It analyzed Wnt/beta-catenin signaling changes using target molecule beta-catenin, Dickkopf-1 (Dkk-1) as a negative regulator, and active caspase3 (CC3) as an apoptotic marker.Dex-9Z,11E-CLA is shown to suppress cell viability in three different types of cancer cells, and the most effective concentration is 200 mu m. 9Z,11E-CLA and Dex-9Z,11E-CLA affect the Wnt/beta-catenin signaling pathway negatively in all cell lines. Beta-catenin expression is decreased, Dkk-1 expression is increased, and CC3 positivity is increased significantly compared to the control group. In conclusion, the data suggest that Dex-9Z,11E-CLA suppresses cell viability and causes apoptosis in cells via the Wnt/beta-catenin signaling pathway. Lastly, Dex-9Z,11E-CLA harbors novel anticancer potential and exhibits uncontrolled activation of Wnt/beta-catenin signaling. Dextran-conjugated cis-9(9Z), trans-11(11E) CLA exhibits potent anticancer activity against three distinct cancer cell lines - MDA-MB-231, T98G, and SAOS-2 - by inducing uncontrolled activation of the Wnt/beta-catenin signaling pathway. image