Objective: The objective of the present study was to assess the effect of erbium, chromium: yttrium-scandiumgallium-garnet (Er, Cr:YSGG) laser-activated irrigation (LAI) of NaOCl on the push-out bond strength of furcal perforations repaired with ProRoot mineral trioxide aggregate (MTA) and Biodentine. Background data: Several studies investigated the adhesion of calcium silicate-based cements after exposure to endodontic irrigants, while effect of LAI on bond strength remains to be elucidated. Materials and methods: Bur-cut furcal perforations with standard dimensions were created in 100 extracted human mandibular molars. Teeth were randomly distributed into two groups (n = 50/group) according to the repair material applied: (1) ProRoot MTA or (2) Biodentine. The specimens were further assigned into five subgroups according to the irrigation regimens used over the set materials: (a) distilled water with needle irrigation; (b) 5.25% NaOCl with needle irrigation; (c) distilled water with LAI; (d) 5.25% NaOCl with LAI; and (e) no irrigation (control). Bond strengths of the test materials were assessed by using push-out bond strength test. Results: Biodentine showed significantly higher dislocation resistance than ProRoot MTA (p < 0.05). Laser activation of 5.25% NaOCl and distilled water did not significantly affect the push-out bond strength results (p > 0.05). Conclusions: Biodentine showed higher dislocation resistance than ProRoot MTA as a perforation repair material. Er, Cr: YSGG laser activation of irrigation aqueous solutions had no adverse effect on push-out bond strength of Biodentine and ProRoot MTA.