The effects of di(2-ethylhexyl)phthalate on rat liver in relation to selenium status

Erkekoglu P., ZEYBEK N. D. , Giray B. K. , Rachidi W., Kizilgun M., Hininger-Favier I., ...Daha Fazla

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, cilt.95, sa.1, ss.64-77, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 95 Konu: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1111/iep.12059
  • Sayfa Sayıları: ss.64-77


This study was performed to determine the hepatotoxicity of di(2-ethylhexyl)phthalate (DEHP) in relation to selenium status. In 3-week-old Sprague-Dawley rats, selenium deficiency was induced by a 0.05 selenium mg/kg. A selenium supplementation group was given 1mg selenium/kg diet for 5weeks. Di(2-ethylhexyl)phthalate-treated groups received 1000mg/kg dose by gavage during the last 10days of the experiment. Histopathology, peroxisome proliferation, catalase (CAT) immunoreactivity and activity and apoptosis were assessed. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR1)], superoxide dismutase (SOD), and glutathione S-transferase (GST); aminotransferase, total glutathione (tGSH), and lipid peroxidation (LP) levels were measured. Di(2-ethylhexyl)phthalate caused cellular disorganization while necrosis and inflammatory cell infiltration were observed in Se-deficient DEHP group (DEHP/SeD). Catalase activity and immunoreactivity were increased in all DEHP-treated groups. Glutathione peroxidase 1 and GPx4 activities decreased significantly in DEHP and DEHP/SeD groups, while GST activities decreased in all DEHP-exposed groups. Thioredoxin reductase activity increased in DEHP and DEHP/SeS, while total SOD activities increased in all DEHP-treated groups. Lipid peroxidation levels increased significantly in SeD (26%), DEHP (38%) and DEHP/SeD (71%) groups. Selenium supplementation partially ameliorated DEHP-induced hepatotoxicity; while in DEHP/SeD group, drastic changes in hepatic histopathology and oxidative stress parameters were observed.