A simple, sensitive, and selective square-wave voltammetric method was developed for the determination of pantoprazole. The influence of the nature of the supporting electrolyte solution, pH, modulation amplitude, frequency, and scan increment was examined by square-wave voltammetric method for pantoprazole. The best results were obtained in Britton-Robinson buffer of pH 5.0. The peak currents were measured with hanging mercury drop electrode at -987 mV vs. Ag/ AgCI. The calibration curve for pantoprazole was found as linear at a concentration range from 0.15 to 25.23 mu g mL(-1). The limit of detection and the limit of quantification of pantoprazole were 0.048 mu g mL(-1) and 0.15 mu g mL(-1), respectively. The validation parameters of the proposed method were evaluated. The method was applied to the pharmaceutical formulation and to both spiked plasma and the plasma of patients orally administered pantoprazole. A spectrophotometric method reported in the literature was utilized as a comparison method. There were no significant differences between the results obtained by two methods.