This study presents effects of biomass concentration, dye concentration, agitation time, initial pH and temperature on dye (Methyl Orange) removal by dead fungal biomass of Humicola fuscoatra. Biosorption capacity was found dependent mainly on. dye and adsorbent dosage. Highest adsorption efficiency was achieved with fungal biomass at 0.5g/l. Dye concentration (100 mg/l) was found optimum for maximum dye removal. At acidic pH (3-5) and 30 C, Methyl Orange biosorption increased significantly in first 6 h.