Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells


Martin S., Dudek-Peric A. M. , Maes H., Garg A. D. , Gabrysiak M., Demirsoy S. , ...More

BIOCHEMICAL PHARMACOLOGY, vol.93, no.3, pp.290-304, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 3
  • Publication Date: 2015
  • Doi Number: 10.1016/j.bcp.2014.12.003
  • Title of Journal : BIOCHEMICAL PHARMACOLOGY
  • Page Numbers: pp.290-304

Abstract

Vemurafenib (PLX4032), an inhibitor of BRAF(V600E), has demonstrated significant clinical anti-melanoma effects. However, the majority of treated patients develop resistance, due to a variety of molecular mechanisms including MAPK reactivation through MEK. The induction of a cancer cell death modality associated with danger-signalling resulting in surface mobilization of crucial damage-associated-molecular-patterns (DAMPs), e.g. calreticulin (CRT) and heat shock protein-90 (HSP90), from dying cells, is emerging to be crucial for therapeutic success. Both cell death and danger-signalling are modulated by autophagy, a key adaptation mechanism stimulated during melanoma progression. However, whether melanoma cell death induced by MAPK inhibition is associated with danger-signalling, and the reliance of these mechanisms on autophagy, has not yet been scrutinized.