Effect of low level laser therapy and zoledronate on the viability and ALP activity of Saos-2 cells


Bayram H., Kenar H., Tasar F., Hasirci V.

INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, vol.42, no.1, pp.140-146, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.1016/j.ijom.2012.03.026
  • Title of Journal : INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
  • Page Numbers: pp.140-146

Abstract

A limited number of clinical studies indicate the supportive role of low level laser therapy (LLLT) on medical and/or surgical approaches carried out in treatment modalities for bisphosphonate related necrosis of jaws (BRONJ), the most common side effect of bisphosphonates used to inhibit bone resorption. The purpose of this study was to investigate the effects of LLLT on cell proliferation and alkaline phosphatase (ALP) activity of human osteoblast-like cells (Saos-2) treated with different doses of zoledronate, the most potent bisphosphonate. Saos-2 cells were treated with different concentrations of zoledronate and were irradiated with diode laser (wavelength 808 nm, 10 s, 0.25 or 0.50W). Cell numbers and ALP activity of the cells were determined. LLLT mildly increased the proliferation rate or ALP activity, while zoledronate reduced both. When applied together, LLLT lessened the detrimental effects of zoledronate and improved cell function and/or proliferation. Based on the results of this study, it was concluded that LLLT has biostimulative effects on Saos-2 cells, even after treatment with zoledronate. LLLT may serve as a useful supportive method for BRONJ treatment through enhancement of healing by osteoblasts.