In this study, we aimed to determine the daily activity patterns and seasonal activity variations of the Eurasian lynx in different habitats (forests and open lands) in a geographical region where there are relatively few data on its ecological characteristics. Survey effort totaled 10 102 camera trap days, with 24 camera trap stations covering an area of approximately 650 km2. Our results showed no significant differences in the habitat preference of the lynx throughout the entire study area or between seasons. The crepuscular and nocturnal activity preferences of the lynx were similar to those of the wolf, red fox, and the European hare. The lynx's daily activity pattern peaked during 20:00–22:00 and 04:00–06:00 hours, and did not show significant variation between seasons. Kernel density estimation was used in order to reveal the temporal overlap of other carnivore and herbivore species with the lynx. The highest temporal overlap (coefficient of overlapping: 0.90) was seen with the European hare. Our findings also indicated higher temporal overlaps with the wolf, red fox, wild boar, and brown bear, and lower overlaps with the red deer, Southwest Asian badger, and Martes spp., which improved understanding of their interactions and co-existence with the lynx.