The effect of different obturation methods on sealer penetration alongside apically separated rotary nickel-titanium instruments: A confocal laser scanning microscopy study

Aktemur Turker S., UZUNOĞLU ÖZYÜREK E., Tek V.

MICROSCOPY RESEARCH AND TECHNIQUE, vol.83, no.6, pp.720-726, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 83 Issue: 6
  • Publication Date: 2020
  • Doi Number: 10.1002/jemt.23461
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.720-726
  • Keywords: confocal laser scanning microscope, obturation technique, sealer penetration, separated instruments, ROOT-CANAL SEALER, DENTINAL TUBULES, RESISTANCE, THICKNESS, INTERFACE, FRACTURE, IMPACT
  • Hacettepe University Affiliated: Yes


The effects of different obturation techniques on calcium silicate-based sealer penetration in the presence of apically separated rotary files were evaluated. Forty-eight extracted mandibular incisors were used. ProTaper F2 rotary files were separated at the apical thirds. Samples were divided into four groups (n = 12) according to obturation technique used: (a) cold lateral compaction (CLC); (b) single cone; (c) bulk-fill (BF) without a core material; and (d) thermoplastic injection (TI). Specimens were sectioned horizontally at 1 and 3 mm from the apex and studied using a confocal scanning laser microscope. The maximum tubule penetration depth and percentage of penetration were measured. Data were statistically analyzed using parametric and nonparametric tests with a significance level of 5%. Regarding penetration depth, a significant difference was found at 1 mm (p < .05), while no significant difference was found at 3 mm (p > .05). At the 3 mm level, all of the obturation techniques showed similar penetration depths. Regarding penetration percentage, the values of the CLC and TI groups were statistically less when compared with the BF group at 1 and 3 mm levels, respectively (p < .05). Under the limitations of this in vitro study, results suggest that the obturation technique may present a significant effect on sealer penetration.