A Novel Chromatographic Media: Histidine-Containing Composite Cryogels for HIgG Separation from Human Serum


Baydemir G., ODABAŞI M.

AFFINITY CHROMATOGRAPHY: METHODS AND PROTOCOLS, 3RD EDITION, vol.1286, pp.221-231, 2015 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1286
  • Publication Date: 2015
  • Doi Number: 10.1007/978-1-4939-2447-9_18
  • Journal Name: AFFINITY CHROMATOGRAPHY: METHODS AND PROTOCOLS, 3RD EDITION
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.221-231

Abstract

Histidine-containing microspheres (HCM) with 2 mu m in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.