A Novel Chromatographic Media: Histidine-Containing Composite Cryogels for HIgG Separation from Human Serum

Baydemir, Gozde; ODABAŞI, MEHMET

doi:10.1007/978-1-4939-2447-9_18

A Novel Chromatographic Media: Histidine-Containing Composite Cryogels for HIgG Separation from Human Serum

Atıf İçin Kopyala

Baydemir G., ODABAŞI M.

AFFINITY CHROMATOGRAPHY: METHODS AND PROTOCOLS, 3RD EDITION, cilt.1286, ss.221-231, 2015 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 1286
Basım Tarihi: 2015
Doi Numarası: 10.1007/978-1-4939-2447-9_18
Dergi Adı: AFFINITY CHROMATOGRAPHY: METHODS AND PROTOCOLS, 3RD EDITION
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.221-231
Hacettepe Üniversitesi Adresli: Evet

Özet

Histidine-containing microspheres (HCM) with 2 mu m in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.