Nucleoside Isolation Performance of Ti4+/Zr4+ Immobilized Polydopamine Coated, Monodisperse-Porous Titania Microbeads


HAMALOĞLU K. Ö.

JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, cilt.30, sa.8, ss.2863-2871, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Konu: 8
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10904-019-01431-6
  • Dergi Adı: JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
  • Sayfa Sayıları: ss.2863-2871

Özet

A Ti4+/Zr4+ ion immobilized sorbent was developed by starting from monodisperse-porous titania microbeads, for the isolation of nucleosides via immobilized metal affinity chromatography (IMAC). For the synthesis of IMAC sorbent, the monodisperse-porous titania microbeads were coated with a polydopamine (PDA) layer. PDA coated titania microbeads were then interacted with TiCl4 or ZrOCl2 solution for the immobilization of Ti4+ or Zr4+ ions onto the PDA layer. Five nucleosides (adenosine, uridine, cytidine, thymidine and guanosine) were individually adsorbed onto Ti4+ or Zr4+ immobilized titania microbeads in aqueous buffer in a pH range of 4-9. The desorption of nucleosides was performed in an aqueous formic acid solution. The nucleoside isolation performance of Ti4+ immobilized sorbent was also compared with Zr4+ immobilized sorbent, PDA coated titania and bare titania microbeads. The highest equilibrium adsorptions and the highest desorption yields were obtained with Ti4+ immobilized sorbent for all nucleosides. It was demonstrated that pH 7 was found as the most appropriate for the adsorption of all nucleosides onto Ti4+ immobilized sorbent, which was also close to the physiological pH of urine. The highest equilibrium adsorption was observed with adenosine as 12.67 mg adenosine/g Ti4+ immobilized sorbent. Almost quantitative desorption yields were achieved with all nucleosides adsorbed in a wide range of pH onto Ti4+ immobilized sorbent. With respect to the similar sorbents developed previously, we believe that the proposed sorbent is a promising candidate for being a stationary phase particularly for the construction of micro-column based IMAC systems used in the continuous isolation of nucleosides.