High test retest reproducibility is essential in tests used for monitoring athletic performance. The purpose of this study was to establish the reproducibility (R) and validity (V) of Hacettepe Intermittent Jumping Test (HIJT) applied in order to determine physiological and mechanical changes during maximal intermittent jumping exercise (5*15 s maximal jumping and 60 s passive recovery between series). Subjects were measured 3 times on separate days to test the R and once in random order to test the V of the HIJT. Subjects performed HIJT on a platform with their hands on their waist. During the study, total and mean power outputs, fatigue index, rest and maximal heart rate were calculated. Three different testing groups (n = 34) joined the R study while only one of these groups joined the V study in which subjects jumped for a minute (60CJT) with their maximum effort. During this stage blood samples were taken from the earlobes of subjects, at the beginning and at the end of the test. A significant correlation was found among tests of different groups on different days (p < 0.05) and HIJT gave similar results during different trials (p > 0.05). The relationships between HIJT and 60CJT were significant (p < 0.05). Based on correlational analysis HIJT demonstrated satisfactory R and V and may thus be used both under laboratory and field conditions to evaluate anaerobic fitness.