Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles

Cetin M., Aktas Y., Vural İ., Capan Y., Dogan L. A., Duman M., ...More

DRUG DELIVERY, vol.14, no.8, pp.525-529, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 8
  • Publication Date: 2007
  • Doi Number: 10.1080/10717540701606483
  • Journal Name: DRUG DELIVERY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.525-529
  • Hacettepe University Affiliated: Yes

Abstract

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.