Synthesis of molecularly imprinted magnetic nanoparticles for selective cytidine adsorption
SEPARATION SCIENCE PLUS, vol.4, no.3, pp.147-156, 2021 (ESCI, Scopus)
- Publication Type: Article / Article
- Volume: 4 Issue: 3
- Publication Date: 2021
- Doi Number: 10.1002/sscp.202000089
- Journal Name: SEPARATION SCIENCE PLUS
- Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
- Page Numbers: pp.147-156
- Keywords: adsorption, cytidine, magnetic nanoparticles, molecularly imprinted polymers, NUCLEOTIDES, RECOGNITION, NUCLEOSIDES, POLYMER
- Hacettepe University Affiliated: Yes
Abstract
Magnetic molecularly imprinted nanoparticles were fabricated for cytidine adsorption. Molecularly imprinted polymers were prepared by complexing N-methacryloyl-l-histidine/Cu(II) and cytidine as a template molecule in a highly cross-linked polymer. Magnetic molecularly imprinted nanoparticles were tested in batch experiments with aqueous samples of cytidine, and several parameters were investigated. The N-methacryloyl-l-histidine/Cu(II) complex was selected to obtain a rebinding of cytidine on the magnetic nanoparticles. The template molecule was removed using 1.0 M NaCl as a desorption agent. The adsorption of cytidine was optimal in a buffer solution of pH 7.4 for 120 min with adsorption capacity of 39.77 mg/g. The imprinted polymer-based magnetic nanoparticles were selective toward cytidine as compared to the analogs (uridine and thymidine). The adsorption fitted a Langmuir isotherm model with an R-2 value of 0.9861 and the Q(max) value of 43.86 mg/g for the magnetic molecularly imprinted nanoparticles.