Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste

Asliyuce, SEVGİ; Mattiasson, Bo; Mamo, Gashaw

doi:10.1016/j.jchromb.2015.12.060

Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, cilt.1021, ss.204-212, 2016 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 1021
Basım Tarihi: 2016
Doi Numarası: 10.1016/j.jchromb.2015.12.060
Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.204-212
Hacettepe Üniversitesi Adresli: Evet

Özet

Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-L-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9 mg of Protein G per g of the cryogel. (C) 2015 Elsevier B.V. All rights reserved.