Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste


Asliyuce S. , Mattiasson B., Mamo G.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol.1021, pp.204-212, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1021
  • Publication Date: 2016
  • Doi Number: 10.1016/j.jchromb.2015.12.060
  • Title of Journal : JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Page Numbers: pp.204-212

Abstract

Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-L-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9 mg of Protein G per g of the cryogel. (C) 2015 Elsevier B.V. All rights reserved.