Molecularly imprinted ligand-exchange recognition assay of DNA by SPR system using guanosine and guanine recognition sites of DNA


Diltemiz S. E. , DENİZLİ A. , Ersoez A., SAY R.

SENSORS AND ACTUATORS B-CHEMICAL, cilt.133, sa.2, ss.484-488, 2008 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 133 Konu: 2
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.snb.2008.03.007
  • Dergi Adı: SENSORS AND ACTUATORS B-CHEMICAL
  • Sayfa Sayıları: ss.484-488

Özet

Molecular imprinting polymer (MIP) as a recognition element for sensors are increasingly of interest and MIP-SPR have started to appear in the literature. In this Study, we have proposed a novel surface imprinting method for DNA recognition and determination by methacryl-amidohistidine-platinum(II) [MAH-Pt(II)] as a new metal-chelating monomer via metal coordination-chelation interactions and guanosine/guanine templates of DNA. The study includes the measurement of binding interaction of molecular imprinted Surface plasmon resonance (SPR) sensor via ligand interaction and recognition selectivity studies of guanosine and guanine imprinted polymers with respect to guanine and dsDNA. Adsorption isotherms of adenosine, guanosine, guanine, adenine, ssDNA and dsDNA were plotted and affinity constants for guanosine and guanine imprinted films were also calculated. (C) 2008 Elsevier B.V. All rights reserved.