Generating somatic mosaicism with a Cre recombinase - microsatellite sequence transgene


Akyol A. , Hinoi T., Feng Y., Bommer G. T. , Glaser T. M. , Fearon E. R.

NATURE METHODS, cilt.5, ss.231-233, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 5 Konu: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1038/nmeth.1182
  • Dergi Adı: NATURE METHODS
  • Sayfa Sayıları: ss.231-233

Özet

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.