Generating somatic mosaicism with a Cre recombinase - microsatellite sequence transgene

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Akyol A., Hinoi T., Feng Y., Bommer G. T., Glaser T. M., Fearon E. R.

NATURE METHODS, vol.5, no.3, pp.231-233, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1038/nmeth.1182
  • Journal Name: NATURE METHODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.231-233
  • Hacettepe University Affiliated: No

Abstract

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.