Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography

Aydogan C., YILMAZ F., DENİZLİ A.

JOURNAL OF SEPARATION SCIENCE, cilt.36, sa.11, ss.1685-1692, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 11
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/jssc.201300089
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1685-1692
  • Anahtar Kelimeler: Capillary column, Cation exchange, Hydrophobic monolith, Nano LC, Vinyl phenyl boronic acid, IMMOBILIZED ENZYME REACTORS, LESS COMMON APPLICATIONS, CAPILLARY ELECTROCHROMATOGRAPHY, STATIONARY PHASES, POLYMETHACRYLATE MONOLITHS, AFFINITY-CHROMATOGRAPHY, POLYMER MONOLITHS, ANION-EXCHANGE, COLUMNS, SEPARATION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In this study, vinyl phenyl boronic acid modified lauryl methacrylate-based monolithic column was successfully prepared for cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins in nano LC. The polymeric mixture consisted of lauryl methacrylate, vinyl phenyl boronic acid as cation exchanger, ethylene dimethacrylate as cross-linker, polyethylene glycol and methanol as binary porogenic solvent, and azobisisobutyronitrile as initiator. The resulting monolith showed good permeability and mechanical stability. Different ratios of monomer and porogens were used for optimizing the properties of the column. The monolithic column performance with respect to hydrophobic and cation exchange interactions was assessed by the separation a series of alkyl benzenes and anilines, respectively. cis-Diol-containing compounds such as phenols were also utilized to evaluate the retention behaviors of the vinyl phenyl boronic acid modified monolithic column. The monolithic column showed cation exchange interactions in the separation of aniline compounds. Theoretical plate number up to 52 000 plates/m was successfully achieved. The prepared monolith was further applied to the proteins with different acetonitrile content.