Requirements for successful mitochondrial transplantation


Kubat G. B. , Ulger O., AKIN Ş.

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2021 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Review
  • Publication Date: 2021
  • Doi Number: 10.1002/jbt.22898
  • Journal Name: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE
  • Keywords: mitochondria dysfunction, mitochondrial isolation, mitochondrial transplantation, FUNCTIONAL MITOCHONDRIA, MYOCARDIAL PROTECTION, STEM-CELLS, REPERFUSION, INTERNALIZATION, DYSFUNCTION, RESCUE, INJECTION, APOPTOSIS, RECOVERY

Abstract

Maintenance of mitochondrial oxidative phosphorylation capacity and other mitochondrial functions are essential for the prevention of mitochondrial dysfunction-related diseases such as neurodegenerative, cardiovascular, and liver diseases. To date, no well-known treatment modality has been developed to prevent or reduce mitochondrial dysfunction. However, a novel approach that transplants fully functional mitochondria directly into defective cells has recently caught the attention of scientists. In this review, we provide an overview of the cell/tissue source of the mitochondria to prompt cell regeneration or tissue repair in vitro and in vivo applications. The animal and human models entail that effective procedures should be used in the isolation and confirmation of mitochondrial membrane potential and function. We believe that these procedures for mitochondrial transplantation for tissue or cell culture will confirm intact, viable, and free from contamination isolated mitochondria from the appropriate sources.