The effects of exercise duration on adrenal HSP72/73 induction in rats


Demirel H., POWERS S., NAITO H., TUMER N.

ACTA PHYSIOLOGICA SCANDINAVICA, vol.167, no.3, pp.227-231, 1999 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 167 Issue: 3
  • Publication Date: 1999
  • Doi Number: 10.1046/j.1365-201x.1999.00609.x
  • Journal Name: ACTA PHYSIOLOGICA SCANDINAVICA
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.227-231
  • Keywords: endurance exercise, heat shock proteins, HSP70, stress proteins, HEAT-SHOCK PROTEIN, STRESS PROTEINS, EXPRESSION, TRANSLOCATION, RESPONSES, CORTEX

Abstract

This study investigated the effects of varying durations of exercise training on heat shock proteins 72 and 73 (HSP72/73) induction in the rat adrenal gland. Female Sprague-Dawley rats (120 days old) were assigned to either a sedentary control group (C) or one of the three endurance exercise training groups. Trained animals ran on a treadmill at approximate to 75% VO2max for 10 weeks (4-5 days week(-1)) at one of the three different exercise durations (30 min day(-1) = T30, 60 min day(-1) = T60, or 90 min day(-1) = T90). All durations of exercise training resulted in an increase in the HSP72 levels (P < 0.0001). The magnitude of the training-induced elevation in the HSP72 levels in the adrenal gland increased as a function of the training duration (approximate to 60-fold increase in T90; approximate to 40-fold increase in T60; and approximate to 15-fold increase in T30). Longer exercise durations (> 60 min day(-1)), also resulted in small but significant increase in HSP73 level (approximate to 1.2-fold increase in both T60 and T90; P < 0.05). These results demonstrate that the physiological stress created by chronic treadmill running can induce both HSP72 and HSP73 in rat adrenal gland. Increased levels of adrenal HSP72/73 expression during rigorous exercise, may be one of the adaptive mechanisms of the adrenals to cope with an increased dose of stress.