A PRACTICAL METHODOLOGY FOR IgG PURIFICATION VIA CHITOSAN BASED MAGNETIC NANOPARTICLES


Kavaz D., Odabas S., DENKBAŞ E. B., Vaseashta A.

DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES, vol.7, no.3, pp.1165-1177, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 3
  • Publication Date: 2012
  • Journal Name: DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1165-1177
  • Hacettepe University Affiliated: Yes

Abstract

The objective of this investigation is to develop a simple, rapid, and practical separation system for IgG separation using magnetic chitosan nanoparticles. Magnetic nanoparticles were prepared by co-precipitation method and coated with a biocompatible polymer chitosan by varying molecular weights. Morphological analyses of magnetic chitosan nanoparticles were investigated by Scanning Electron Microscope (SEM) and Zeta-Sizer. Chemical structure of the nanoparticles was analyzed by Fourier Transform Infra-Red Spectroscopy (FTIR) and magnetic properties were investigated using Electron Spin Resonance (ESR) and Vibrating Sample Magnetometer (VSM). For the purification of IgG, a well-known protein Concanavalin A (Con-A), having a strong affinity to the IgG molecules, was immobilized onto magnetic chitosan nanoparticles. IgG purification was performed using a batch separation system made locally. It was observed that IgG can easily be purified from fresh human plasma with high efficacy, using a simple and inexpensive system prepared locally. Nanoparticle size, IgG concentrations and the Con-A concentrations were investigated to get an optimum separation efficiency.