The aim of this study was to compare the efficacy of Mtwo, Pro Taper retreatment files and Hedstrom files for the retreatment of root canals filled with Bee Fill 2 in 1 and cold lateral compaction techniques. 63 single-rooted human teeth were prepared with Mtwo rotary files to size 35/.04 and assigned into three groups (n=21/group). (1) Bee Fill 2 in1/2Seal, (2) Bee Fill 2 in 1/AH26 and (3) laterally-compacted gutta-percha/AH26. The specimens were subgrouped according to the file system used to remove root fillings (n=7/subgroup): 1. Mtwo retreatment file, 2. Pro Taper retreatment file, 3. Hedstrom file. Roots were split longitudinally and photographed. The time required for removal of root canal fillings and the areas of residual root filling materials were compared statistically (p<0.05). The maximum amount of remnant was observed in the Mtwo group for removal of BeeFill 2 in1/AH26. ProTaper retreatment files required less time to remove root filling material than Mtwo retreatment files and Hedstrom files (p<0.05). Among the tested removal systems. ProTaper retreatment files may offer the best efficiency-speed combination.