Co-circulation of West Nile virus and distinct insect-specific flaviviruses in Turkey

ERGÜNAY K., Litzba N., Brinkmann A., GÜNAY F., Sarikaya Y., Kar S., ...More

PARASITES & VECTORS, vol.10, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 10
  • Publication Date: 2017
  • Doi Number: 10.1186/s13071-017-2087-7
  • Journal Name: PARASITES & VECTORS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: West Nile virus, Flavivirus, Insect-specific, Biosurveillance, Mosquito, Turkey, FIELD-COLLECTED MOSQUITOS, SURVEILLANCE, ALIGNMENT, SEQUENCE
  • Hacettepe University Affiliated: Yes

Abstract

Background: Active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. This study was undertaken to investigate the circulation of flaviviruses. Mosquitoes were collected from 58 locations in 10 provinces across the Aegean, Thrace and Mediterranean Anatolian regions of Turkey in 2014 and 2015. Following morphological identification, mosquitoes were pooled and screened by nested and real-time PCR assays. Detected viruses were further characterised by sequencing. Positive pools were inoculated onto cell lines for virus isolation. Next generation sequencing was employed for genomic characterisation of the isolates.