Impaired Placentation and Early Pregnancy Loss in Patients with MTHFR Polymorphisms and Type-1 Diabetes Mellitus

Gurbuz R. H., ATİLLA P., ÖRGÜL G., TANAÇAN A., Dolgun A., Cakar A. N., ...Daha Fazla

FETAL AND PEDIATRIC PATHOLOGY, cilt.38, sa.5, ss.376-386, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 5
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/15513815.2019.1600623
  • Dergi Adı: FETAL AND PEDIATRIC PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.376-386
  • Anahtar Kelimeler: MTHFR gene polymorphisms, type 1 diabetes mellitus, leukemia inhibitory factor, insulin like growth factor 1, beclin 1 protein, LEUKEMIA INHIBITORY FACTOR, MATERNAL-FETAL INTERFACE, GENE POLYMORPHISMS, APOPTOSIS, IMPLANTATION, HOMOCYSTEINE, AUTOPHAGY, OUTCOMES, IGF-1
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Objective: To evaluate the impact of type-1 diabetes mellitus (DM) and methylenetetrahydrofolate reductase (MTHFR) polymorphisms on impaired placentation leading to early pregnancy loss. Methods: Miscarriage materials were obtained from eight pregnant women with type-1 DM without MTHFR polymorphism, eight with MTHFR polymorphisms without type-1 DM, and eight controls with neither DM nor MTHFR polymorphisms. Insulin-like growth factor-1 (IGF-1), leukemia inhibitory factor (LIF), and Beclin-1 expression were assessed to evaluate placentation. Results: Cytoplasmic LIF, IGF-1, and Beclin-1 expression were decreased in the superficial and glandular epithelial cells of the decidua in both study groups. LIF expression was increased in interstitial trophoblasts in the MTHFR group. IGF-1 expression was decreased in the decidual cells and interstitial trophoblasts in both study groups, while the decrease in stromal cells was noted only in type-1 DM group. Beclin-1 expression was increased in interstitial and villous trophoblasts in both study groups. Conclusion: The expression of IGF-1, LIF, and Beclin-1 are altered in both the decidua and the trophoblasts in pregnancies of women with type-1 DM and MTHFR polymorphisms, compared to normal pregnancies undergoing (elective) terminations.