Development and in-vitro characterization of 1-cysteine loaded alginate beads for oral delivery


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ÖZTÜRK ATAR K.

JOURNAL OF RESEARCH IN PHARMACY, vol.26, no.1, pp.210-218, 2022 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.29228/jrp.119
  • Journal Name: JOURNAL OF RESEARCH IN PHARMACY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.210-218
  • Keywords: Ca-alginate bead, sodium alginate, oral drug delivery, 1-cysteine, controlled release, ACETYL-L-CYSTEINE, CALCIUM ALGINATE, SUSTAINED DELIVERY, N-ACETYLCYSTEINE, CHITOSAN, RELEASE, OXIDATION, ACID, MECHANISMS, KINETICS
  • Hacettepe University Affiliated: Yes

Abstract

The purpose of this work is to develop a biodegradable calcium alginate (Ca-alginate) bead formulation to provide controlled release of 1-cysteine (Cys) amino acid for oral drug delivery. Ca-alginate bead formulations were prepared successfully and reproducibly using calcium chloride as a cross-linking agent. The beads were then evaluated in terms of morphology, particle size, swellability, thermal behaviors, encapsulation efficiency, and in-vitro drug release at pH 1.2 and pH 7.4. The size of the beads was measured between 2.23 +/- 0.11 and 2.41 +/- 0.12 mm. In terms of thermal analysis, differential scanning calorimetry was utilized to ensure the encapsulation of Cys into bead formulation. Cys encapsulation was determined as 79.49 %+/- 1.12. Swelling index of blank beads were found to be in a range of 0.31-0.35 in HCl solution at pH 1.2; 0.98-1.08 in PBS buffer at pH 7.4. Alginate beads exhibited controlled release, followed the Weibull model which is consistent with the swelling pattern. The physico-chemical properties of the developed formulation indicate that the formulation is suitable for oral Cys delivery.