Gadolinium-loaded polychelating polymer-containing cancer cell-speciflc immunoliposomes

Erdogan S., Roby A., Sawant R., Hurley J., Torchilin V.

JOURNAL OF LIPOSOME RESEARCH, cilt.16, sa.1, ss.45-55, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/08982100500528784
  • Dergi Adı: JOURNAL OF LIPOSOME RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.45-55
  • Anahtar Kelimeler: cancer imaging, MRI, Gd, liposome, polychelating amphiphilic polymers, cancer-specific monoclonal antibody, TARGETED DELIVERY, CONTRAST AGENTS, LABELED LIPOSOMES, DIAGNOSTIC AGENTS, IMAGING AGENTS, TUMOR-CELLS, IMMUNOMICELLES, ANTIBODIES, MICELLES, CARRIERS
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Liposome loading with Gd via the membrane-incorporated polychelating amphiphilic polymers (PAPs) significantly increases the Gd content and relaxivity (T1 parameter) of PEGylated liposomes, which can be used as contrast agents for magnetic resonance imaging (MRI). Here, we demonstrate that such Gd-containing liposomes can be additionally modified with the monoclonal anticancer antibody 2C5 (mAb 2C5) possessing the nucleosome(NS)-restricted specificity via the PEG spacer. Liposome-bound antibody preserves its specific activity (ELISA) and such Gd-loaded PEGylated 2C5-immunoliposomes specifically recognize various cancer cells in vitro and target an increased amount of Gd to their surface compared to antibody-free Gd-liposomes or Gd-liposomes modified with tumor nonspecific antibody. Gd-loaded cancer cell-targeted immunoliposomes may represent promising agents for enhanced tumor MRI.