In vivo biocompatibility studies of poly(n-vinyl 2-pyrrolidone/itaconic acid) hydrogels synthesized by gamma-rays

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Ozdemir S., Ozdemir E., Tunca R., Haziroglu R., Sen M., Kantoglu O., ...Daha Fazla

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, cilt.208, ss.395-399, 2003 (SCI-Expanded) identifier identifier

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In this study, poly(n-vinyl 2-pyrrolidone/itaconic acid) hydrogels have been synthesized by gamma-rays in different compositions and their biocompatibility have been investigated as in vivo and some biochemical parameters of mice serum and histology of their tissues have been examined. By these purposes, poly(n-vinyl 2-pyrrolidone/itaconic acid) (P(VP/ IA)) hydrogels were implanted to hypersensitive mice (BALB/c). One and a half months after implantation, hydrogel implanted animals were sacrificed by ether anesthesia and the area hydrogel contacted with tissue was investigated by light microscope for histopathological identification of the tissue. Then the total immunoglobulin E (IgE) level was determined by ELISA. Differential white cell count was also made to better understanding of reaction between hydrogel and tissue. These poly(n-vinyl 2-pyrrolidone/itaconic acid) hydrogels can be directly used as biomedical materials. (C) 2003 Elsevier B.V. All rights reserved.

Adsorption and desorption capacity of hydrogel depends on the IA content in the hydrogel. The hydrophilic groups of the hydrogels increase with an increase in IA content. Thus, the swelling ratio of hydrogels increases. This increase results with an increment or a decrement in the adsorption or desorption capacity of hydrogel. For the practical biomedical application of the hydrogel, it is therefore important to know that the influence of IA content on the biocompatibility of PVP/IA hydrogel. For this reason, poly(n-vinyl 2-pyrrolidone/itaconic acid) hydrogels have been synthesized by γ-rays in different compositions and their biocompatibility have been investigated as in vivo and some biochemical parameters (UN, ALT, ELISA) of mice serum and histology of their tissues have been examined. UN, ALT, ELISA and histopathological analyses result can be attributed to a normal defense mechanism of the tissue against foreign body. It shows that poly(n-vinyl 2-pyrrolidone/itaconic acid) hydrogels can be directly used as biomedical materials.