Confocal reflectance microscopy for metal and lipid nanoparticle visualization in the brain

ÇAKIR AKTAŞ C., ERDENER Ş. E., Teke B., Pehlivan S. B., ZEYBEK N. D., TAŞKIRAN SAĞ A., ...More

NANOMEDICINE, vol.17, pp.447-460, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17
  • Publication Date: 2022
  • Doi Number: 10.2217/nnm-2021-0350
  • Journal Name: NANOMEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, BIOSIS, Biotechnology Research Abstracts, EMBASE, MEDLINE
  • Page Numbers: pp.447-460
  • Keywords: brain, confocal, fluorescence, glioblastoma, lipid, metal, nanoparticles, reflectance, silver, stearylamine, DRUG-DELIVERY, SILVER NANOPARTICLES, TISSUE DISTRIBUTION, IMAGING TECHNIQUES, IN-VITRO, BARRIER, PHARMACOKINETICS, TRANSPORT, SKIN, SLN
  • Hacettepe University Affiliated: Yes

Abstract

Plain language summary Nanoparticles (NPs) are extremely small materials utilized in the healthcare sector mainly for the delivery of drugs into tissues that are not easily accessible with regular pharmaceuticals. One such tissue is the brain, which has a barrier between it and the bloodstream that prevents the passage of most drugs. For NP research, the successful entry of NPs into target tissues must be demonstrated, but this is complicated by the small size and weak labeling of NPs. In this article, the authors demonstrate a low-cost, complementary microscopy technique that is readily available in most biological research laboratories and that can be used to detect and analyze the entry of different NP types into brain tissue and their uptake by brain tumor cells. These data create new opportunities for research on NP-assisted drug delivery to the central nervous system.